Production of ß-D-Galactosidase from Whey Using Kluyveromyces marxianus

Electroporation of Kluyveromyces marxianus and -D-galactosidase Extraction

Nowadays electroporation is well established as a method for increasing the permeability of biological membranes aiming to include some kind of molecule inside cells (anticancer drugs, for example) or to extract molecules from cells (enzymes, DNA, etc.). The permeabilized cells show a distribution of hydrophilic pores with diameters of several nanometers in the regions where the induced transme...

Studies on the Optimiization of Biomass Production Using Kluyveromyces Marxianus Isolated from Paneer Whey

In the present study Kluyveromyces marxianus isolated from paneer whey was employed for biomass production. Various parameters like pH, temperature and nitrogen source were optimized for maximum biomass yield. The biomass yield was studied to vary with different nitrogen sources, pH and temperature. Ammonium sulphate, ammonium nitrate, beef extract and yeast extract were used as nitrogen source...

Production and purification of lactase of Kluyveromyces fragilis from whey

Kinetics and Regulation Studies of the Production of -Galactosidase from Kluyveromyces marxianus Grown on Different Substrates

Lactose-intolerance is manifested in 50 % of the world’s population. This can be remediated by removing lactose from the diet or converting it into glucose and galactose with -galactosidase (EC 3.2.1.23). In this work, batch production of this enzyme in the presence of lactose, galactose, cellobiose, xylose, arabinose, sucrose and glucose was investigated using Kluyveromyces marxianus in shake ...

Production of inulinase from Kluyveromyces marxianus using dahlia tuber extract

Various carbon sources were evaluated for production of inulinase by yeast, Kluyveromyces marxianus MTCC 3995. Highest inulinase activity was observed with Dahlia extract (25.3 nkat mL(-1)) as carbon source. The enzyme activity was 1.4 folds higher than that observed in media containing pure chicory inulin (17.8 nkat mL(-1)). The yeast showed good growth on a simple medium containing dahlia ext...